Lab Report Osmosis

The effect of osmosis on false cellular phones with contrastive concentrations of saccharose Alex McRae Biology 120-902 Grand Valley State University 1 Campus Drive Allendale, MI 49401 emailprotected gvsu. edu Abstract In this study, we tested the validity of osmosis in factitious beast cells. Osmosis is the diffusion of free urine crossways a membrane. The purpose of the study was to calculate the point of osmosis in slushy cells containing dissimilar concentrations of sucrose and urine.We studied the ordain of osmosis in artificial cells by creating louver different dialysis come outs with different concentrations of both sucrose and body of peeing and calculating the accumulative sort in encumbrance ever 10 proceedings for 90 minutes. Our solvings for the artificial cells showed different concentrations travel from high to wretched concentrations- through hypotonic proceeding or hypertonic movement. Introduction The main purpose of this topic is to assess the number of change with osmosis for different concentrations of sucrose in artificial cells.Since the sympathetic body is composed of trillions of cells that contain roughly 85% of body of water, makes osmosis a very important concept (Carmichael, Grabe and Wenger). The forces that affect osmosis are the concentrations of solutes environ the cell or inside of the cell. Water will then move across the cell membrane and create a balance of water among the cell and its environment (Reece et al. 133).In position to calculate the average invest of change for our artificial cells, we must understand tonicity as the ability of a nearby solution to cause a cell to lose or gain water, depending on its concentration of non-penetrating solutes relative to solutes inside the cell (Reece et al. 133). The dialysis bags utilize in this sample have membranes which are selectively permeable, which only suffers particles specifically small enough to pass through (Carmichael, Grabe and We nger).In a hypotonic solution, water goes into the cell because the solute is more than concentrated inside the cell, while in a hypertonic solution, water moves out of the sell because the solute is more concentrated extracurricular of the cell. We are testing the effect of osmosis on different concentrations of artificial cells by calculating the cumulative change in weight and the reverse cumulative changes in weight and by determining whether a solution is hypertonic, hypotonic or isotonic. We predicted that a dialysis bag prop hydrant water in a beaker also containing tap water is in an isotonic solution.While 20% sucrose, 40% sucrose and 60% sucrose in beakers containing tap water is considered hypotonic solutions. Lastly the dialysis bag holding tap water in a beaker containing 40% sucrose is a hypertonic solution. This will result in isotonic solutions remaining at the same weight, hypotonic solutions gaining weight and hypertonic solutions losing weight. We tested this by creating the five different dialysis bags with different concentrations of sucrose in order to measure the weight change in grams of the bag after nine 10 minute increments. Methods and MaterialsThis experiment took place on Monday, February 6th, 2011. During this beat, we tested the effects of different sucrose concentrations on the rate of osmosis in artificial cells we made with dialysis tubing. We studied five different dialysis bags containing 10mL of different concentrations of tap water and sucrose. Two contained tap water while three contained different concentrations of sucrose, varying from 20% to 60%. severally bag was placed in a beaker surrounded by either tap water or 40% sucrose. We began the experiment by soaking the dialysis tubes to prepare them for the sucrose concentrations they would be fill up with.Taking from each one bag, two were filled with 10mL of tap water, one filled with 10mL of 20% sucrose, one with 10mL of 40% sucrose and an another(prenomina l) with 10mL of 60% sucrose. Each bag was clamped closed. All the bags were weighed before being placed in their synonymic beakers in order to record their sign weight in grams. The bags were say in their corresponding beakers, all of which contained tap water, except beaker 5 (tap water bag 5 was placed in beaker 5 which instead of holding water, was filled with 40% sucrose) concurrently, recording the time.In the same manner in which the bags were placed in the beakers simultaneously, remove the bags every 10 minutes, and record the weight of each bag. This process should be repeated for at least 90 minutes innate. This data was analyzed by calculating the cumulative change in weight for each dialysis bag. This was done from subtracting the weight of each bag from the initial weight of the bag. Doing so, allows the weight of each bag to be initially zero. For that, we must calculate the corrected cumulative change in weight.For each time interval of 10 minutes, we subtracted the change in weigh of bag 1 (tap water) from the weight of each bag at the specific time measure- this corrected any oscillations. Results The corrected cumulative change in weight overdue to osmosis from different concentrations of sucrose and tap water, are shown in Figure One. This common fig tree shows the weight change in grams for every interval of 10 minutes. use the corrected cumulative change in weight eliminates bag 1 because its average rate of change will always be zero.Below is a table of the bag weights at 10 minute intervals after being tested for an hour old bag Weights (g) Time (min) 1 2 3 4 5 Water 20% 40% 60% water 0 21. 81 20. 30 23. 3 21. 30 19. 22 10 22. 75 26. 94 22. 04 23. 64 18. 42 20 22. 29 26. 91 22. 29 24. 41 17. 95 30 23. 27 29. 33 23. 45 26. 41 16. 60 40 22. 30 29. 84 23. 24 28. 6 15. 61 50 22. 72 36. 63 24. 02 28. 84 14. 75 60 23. 29 31. 20 24. 51 30. 17 14. 05 The purpose of this experiment was to settle down the relationship betwee n concentration gradients and the rates of osmosis. Using the corrected cumulative change, we can monitor the rate of change for each bag, and correlate the rate of change to the rate of osmosis.For bag 2, the slope, or the rate of osmosis was y = 0. 1193x 1. 7293, displaying a slow but obvious increase in weight, or a hypotonic solution, when the solute was more concentrated inside the cell and water moved into the cell. Bag 3 continues to show this trend with a quicker rate of y = 1. 295x 2. 4807, which water enters this bag as a hypotonic solution. Bag 4, which a rate of y = -1. 0586x + 1. 9043, shows a hypertonic solution in which the low concentration solute, causing water inside the dialysis bag, to move out.Although it was expect for for bag 5, which was tap water submersed in 40% sucrose, to be hypertonic, the rate of osmosis was y = 1. 3536x 0. 1679, which demonstrates a hypotonic solution, or water debut the cell, or touching from a high concentration of the solute to a low concentration. These results prove that the counsellor of osmosis does directly affect the rate of osmosis. If the slope go abouts with a negative x value, the solution is indeed a hypertonic solution, that when surrounding a cell will cause the cell to lose water, moving from a high concentration to a lower concentration (Reece et al. 33). The slopes which begin with a positive x value demonstrate a hypotonic solution, which causes a cell to take in water (Reece et al. 133). This shows that the watchfulness of osmosis is direct related to the rate of osmosis, or vice versa. The rate of osmosis ultimately determines the direction of osmosis. Depending on which direction osmosis is going- hypertonic, isotonic or hypotonic, determines the rate of osmosis, or the rate of change for each dialysis bag. Or by the means of our experiment, the direction of osmosis was unyielding by the rate of change in each bag, or the rate of osmosis.Discussion Throughout the study it was concl uded that different concentrations of sucrose are allow different rates and directions of osmosis. The results show that the rate of osmosis is directly related to the direction of osmosis, or vice versa. This proposal does not match with our quantitative prediction. Our results for the artificial cells showed different concentrations moved from high to low concentrations- through hypotonic movement or hypertonic movement however bag 3 with 40% sucrose was expected to be a hypotonic solution, while it was a hypertonic solution.This falsified hypothesis could be due to the explanation that in an animal cell, when a hypertonic solution, the cell experiences crenation. The dialysis tubing creates a theoretical stigma in our experiment because the tubing has a molecular weight contract off of a maximum of 14 kilodaltons, while the average human cell may have a larger or littler molecular weight cut off, allowing the cell to experience different tonicities. In order to obtain more accu rate results, modifications should be made. More drastic concentrations of sucrose in the dialysis tubing should be tested in order to find the extremes of the rate of change for osmosis.The study enhances the present scholarship in this area by exposing osmosis along a free energy gradient. However, other experiments could increase our knowledge about the relationship between concentration gradients and rates. An experiment that includes the idea that the selectively permeable membrane moves, might allow for more accurate results (Patlak and Watters). The qualified location mirrors the volume of each side of the membrane, which affects the total number of particles on each side (Patlak and Watters).Our experiment exposes the ideal tactual sensation that there is no net movement of a solvent and the water is what diffuses across the membrane. Works Cited Carmichael, Jeff, Mark Grabe and Jonathan Wenger. Biology 150 Laboratory Review. University of northwestward Dakota, n. d. Web. 7 Oct. 2011. Patlak, Joseph and Chris Watters. Diffusion and Osmosis. University of Vermont and Middlebury College, 1997. Web. 8 Oct. 2011. Reece, Jane B. , et al. Campbell Biology. San Francisco Pearson Education Inc. , 2005. Print.